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Image Search Results
Journal: bioRxiv
Article Title: Restriction of the growth and biofilm formation of ESKAPE pathogens by caprine gut-derived probiotic bacteria
doi: 10.1101/2023.06.30.546197
Figure Lengend Snippet: The figure depicts representative images of nutrient agar plates from agar well diffusion assay-based antimicrobial activity assay. Each ESKAPE bacterial inoculum was spread onto nutrient agar plates. Subsequently, 10 mm diameter wells were made on the agar plates using 1 ml sterile pipette tip bottoms. Sterile-filtered cell-free culture supernatants (S) and cell lysate (L) of LAB isolates were added to each well and incubated for 24 hours at 37°C in aerobic conditions. The zone of inhibition was recorded with the zone of inhibition scale. Experiments are performed thrice.
Article Snippet:
Techniques: Diffusion-based Assay, Activity Assay, Sterility, Transferring, Incubation, Inhibition
Journal: bioRxiv
Article Title: Restriction of the growth and biofilm formation of ESKAPE pathogens by caprine gut-derived probiotic bacteria
doi: 10.1101/2023.06.30.546197
Figure Lengend Snippet: The biofilm inhibition ability of LAB isolates was assessed using Cell-free culture filtrate (CSF). The assay was performed in 24-well polystyrene plates. ESKAPE pathogens were cultured either in the presence or absence of LAB-CSF and incubated in static conditions at 37 °C for 48 hours. Subsequently, quantitative measurement of biofilm was performed using the standard crystal violet-based assay. The bar diagrams depict the Mean ± SD of the absorbance (A 570nm ) values from triplicate wells. The experiment was performed twice. One-way ANOVA was performed to compare the mean differences of each condition compared to the base value without any CSF (control biofilm, CB). *, p<.05, **, p<.01, ***, p<.001, ****, p<.0001. All LAB-CSF exhibited various degrees of biofilm inhibition on ESKAPE bacteria.
Article Snippet:
Techniques: Inhibition, Cell Culture, Incubation, Crystal Violet Assay, Control, Bacteria